Our state-of-the-art sample preparation stations offer many advantages, enabling you to enhance efficiency, minimize the risk of expensive errors inherent in manual procedures, and automate crucial tasks like pipetting and data analysis. These innovative systems are particularly valuable for applications involving cell preparation, evaluating cell viability, analyzing cultured cells, and performing monoclonal antibody studies.
Features
Essential Features
- These sample preparation stations exhibit exceptional performance, ensuring precise and efficient preparation of different cell types, including cell populations and cryopreserved cells, while optimizing the fluorescence of cells for accurate flow cytometry Sample Preparation and reliable preparation of cells for staining.
- With a strong focus on quality, the Stations deliver consistent and reliable results, maintaining the integrity of cells throughout the preparation process. They enable the preparation of samples with high precision and accuracy while offering robust negative control options to ensure reliable data quality.
- Designed to withstand demanding laboratory environments, the Stations are built to last, providing long-lasting durability. Their sturdy construction and reliable components ensure uninterrupted operation, even with high sample volumes and frequent use, making them a reliable workhorse in the lab.
Applications
Applications
- Our sample preparation stations are instrumental in monoclonal antibody development. They support the binding process by accurately preparing the necessary samples, including antigens and hybridoma cells, for antibody screening and characterization.
FAQs
What samples are used in flow cytometry?
Flow cytometry samples include blood, tissues, bone marrow, lymph nodes, and cell cultures, as well as whole blood samples, tissue digests and particulate samples.
How to prepare cells for flow cytometry?
To prepare cells for flow cytometry, isolate them into a single-cell suspension, adjust cell concentration, stain with fluorochrome-conjugated antibodies, acquire data using a flow cytometer, and analyze the results to identify and quantify cell populations of interest.
What are the key considerations for the preparation of cell line samples?
When preparing cell line samples for flow cytometry, it is important to consider factors such as staining protocol parameters (time, temperature, volume, cell number, antibody amount), proper washing steps, choice of buffers and fixatives, minimizing pipetting errors, and implementing blocking and dead cell discrimination strategies. Additional considerations include tissue digestion for single-cell isolation, gating and staining controls for data analysis, determining optimal cell numbers for the experiment, and maintaining consistency to ensure reproducible results.
What are the key features of quality samples for flow cytometry analysis?
Flow cytometry is an invaluable technique for analyzing and quantifying heterogeneous samples, and ensuring high-quality data relies on key considerations. The critical steps include effective sample preparation, particularly in generating single-cell suspensions, assessing cell viability, and avoiding debris or aggregates. Incorporating appropriate controls like biological, compensation, and FMO controls is crucial, as is employing standardized marker and channel designations for improved reproducibility. Lastly, ensuring instrument quality and optimized data acquisition settings contribute to reliable results, making timing crucial for clinical monitoring and therapeutic interventions.
What is the sample size for flow cytometry?
The sample size for flow cytometry can vary depending on the specific experiment, instrument capabilities, and the number of cells available. In general, flow cytometry can analyze a wide range of sample sizes, from a few cells up to millions of cells.
What are the different types of cell preparation systems that we provide?
We carry different types of sample preparation stations:
- CellMek SPS: It revolutionizes flow cytometry sample preparation optimization by incorporating a refrigerated 53-position liquid antibody carousel that allows reagents to stay onboard while also efficiently managing scheduling without the need to batch by application.
- TQ-Prep workstation: It enhances flow cytometric analysis by standardizing whole blood lysis, optimizing workflows with a reduced need for repeat samples, providing a user-friendly interface with an icon-driven touch screen, and achieving a high sample throughput of up to 32 samples in just 24 minutes for improved cost and labor efficiencies.