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AB108754

Human Anti-Mycoplasma pneumoniae IgA ELISA Kit

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Human Anti-Mycoplasma pneumoniae IgA ELISA Kit is an indirect ELISA for the qualitative detection of IgA class antibodies against Mycoplasma pneumoniae in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Primary Antibodies

AB20704

Anti-Mycoplasma pneumoniae antibody

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Secondary Antibodies

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  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Mycoplasma pneumoniae IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Mycoplasma pneumoniae in Human serum and plasma.

A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgA sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Negative Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Negative Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108754-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Mycoplasma pneumoniae anti-IgA HRP Conjugate", "number":"AB108754-CMP02", "productcode":"" }, { "size":"1 x 2 mL", "name":"Mycoplasma pneumoniae IgA Negative Control", "number":"AB108754-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"Mycoplasma pneumoniae IgA Cut-off Control", "number":"AB108754-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Mycoplasma pneumoniae IgA Positive Control", "number":"AB108754-CMP04", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgA Sample Diluent", "number":"AB108754-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108754-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108754-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108754-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mycoplasma pneumoniae (IgA) Coated Microplate (12 x 8 wells)", "number":"AB108754-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mycoplasma pneumoniae IgA is an immunoglobulin A (IgA) antibody targeting the Mycoplasma pneumoniae bacterium. This protein does not have an alternate common name. Its mass is typically not measured as a standalone protein due to the variability in IgA glycoproteins. IgA expression occurs mainly in mucosal areas including respiratory and gastrointestinal mucosa as it plays an important role in mucosal immunity. Mycoplasma pneumoniae is a small bacterium lacking a cell wall capable of causing respiratory infections particularly in enclosed community settings such as schools.
Biological function summary

The immune system utilizes Mycoplasma pneumoniae IgA to prevent colonization by this bacterium and to neutralize its effect. While not forming part of a large multi-protein complex IgA engages with various immune elements assisting in pathogen clearance. This antibody contributes to immune response modulation and mucosal protection stopping the Mycoplasma pneumoniae bacterial infections from impeding normal physiological function.

Pathways

Interactions involving Mycoplasma pneumoniae IgA align with mucosal immunity and adaptive immune pathways. In these pathways this antibody operates in concert with other immunoglobulins such as IgM and IgG to establish an effective line of defense against bacterial invaders. These immune pathways facilitate the identification and removal of Mycoplasma pneumoniae maintaining respiratory health and preventing persistent infection.

Mycoplasma pneumoniae IgA is strongly connected to respiratory diseases especially atypical pneumonia or walking pneumonia. In this context elevated levels of specific IgA can indicate infection aiding in diagnosis. Furthermore this marker has associations with extrapulmonary manifestations of infection such as skin rashes and joint inflammation. These disease processes may involve other proteins like pro-inflammatory cytokines highlighting IgA’s role in both respiratory and systemic immune responses.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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