AB96951

Goat Anti-Chicken IgY H&L (DyLight® 488) preadsorbed

Name: Goat Anti Chicken (IgY) secondary antibody preadsorbed DyLight® 488 (ab96951)
Brand: Abcam Limited
SKU: AB96951
Price: 295 USD
Availability: InStock

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(6 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Rabbit, Goat, Horse, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Applications

IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Chicken IgY

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111313 PubMed36070687

2022

Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Wei,Li Zhang,Yiqun Zhang,Cody Cooper,Chris Brewer,Chia-Feng Tsai,Yi-Ting Wang,Micah Glaz,Hunter B Wessells,Jianwen Que,Mark A Titus,Vincenzino Cirulli,Adam Glaser,Tao Liu,Nicholas P Reder,Chad J Creighton,Li Xin

Scientific reports 9:5782 PubMed30962493

2019

Uncoupling of in-vitro identity of embryonic limb derived skeletal progenitors and their in-vivo bone forming potential.

Applications

Unspecified application

Species

Unspecified reactive species

Louca Verbeeck,Liesbet Geris,Przemko Tylzanowski,Frank P Luyten

PloS one 14:e0213327 PubMed30943201

2019

YAP/Yorkie in the germline modulates the age-related decline of germline stem cells and niche cells.

Applications

Unspecified application

Species

Unspecified reactive species

Deepthy Francis,Bhavna Chanana,Beatriz Fernandez,Benjamin Gordon,Tiffany Mak,Isabel M Palacios

The Journal of clinical investigation 128:1087-1105 PubMed29431735

2018

Vhl deletion in osteoblasts boosts cellular glycolysis and improves global glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Naomi Dirckx,Robert J Tower,Evi M Mercken,Roman Vangoitsenhoven,Caroline Moreau-Triby,Tom Breugelmans,Elena Nefyodova,Ruben Cardoen,Chantal Mathieu,Bart Van der Schueren,Cyrille B Confavreux,Thomas L Clemens,Christa Maes

Journal of bone and mineral research : the officia 32:2087-2102 PubMed28574598

2017

Integrin-Linked Kinase Regulates Bone Formation by Controlling Cytoskeletal Organization and Modulating BMP and Wnt Signaling in Osteoprogenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Marian Dejaeger,Anna-Marei Böhm,Naomi Dirckx,Joke Devriese,Elena Nefyodova,Ruben Cardoen,René St-Arnaud,Jos Tournoy,Frank P Luyten,Christa Maes

Stroke research and treatment 2013:869327 PubMed23691431

2013

Increased cell fusion in cerebral cortex may contribute to poststroke regeneration.

Applications

IHC

Species

Unspecified reactive species

Alexander Paltsyn,Svetlana Komissarova,Ivan Dubrovin,Aslan Kubatiev

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Chicken IgY H&L (DyLight® 488) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (6)

Product promise