DispenCell™ Single-Cell Dispenser
Compact, automated cell dispenser for fast, easy and gentle single-cell isolation
A simple single-cell dispenser for proof of monoclonality
The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument designed for fast, easy and gentle single-cell isolation. DispenCell integrates seamlessly into your laboratory workflow, with a plug-and-play approach. Flexible and effortless, DispenCell operates equally under sterile conditions in a culture hood, or on a simple benchtop.
Immediate and traceable proof of clonality
DispenSoft, the single-cell analysis software included with DispenCell, provides immediate and traceable proof of clonality. The instrument is fitted with a sensing tip that detects the passage of cells, allowing users to check for proof of clonality immediately after the cells are dispensed. All dispensing data is automatically stored, allowing for easy generation of a clonality report.
As gentle as manual pipetting
DispenCell has unique technology which allows for extremely gentle handling of the cell sample. Cells are exposed to no more pressure than with manual pipetting (less than 0.1 psi). This allows the instrument to preserve cell viability and outgrowth while simultaneously increasing cell deposition efficiency.
Compact
The small footprint of the DispenCell allows it to fit perfectly into any laboratory setting: under a hood to work under sterile conditions, on a bench top for routine seeding, or in a pre-existing automated workflow.
Features of DispenCell™ Single-Cell Dispenser
Proof of clonality
A single-cell analysis software tool provides a traceable proof of clonality report instantly.
Contamination-free
A patented disposable tip ensures clean isolation of single cells and no cross contamination. Certified free from animal products and cytotoxic material.
High cloning efficiency
Unique design ensures gentle dispensing for better viability and cloning efficiency.
Easy to use
Easy to set up, and intuitive with a simple interface. No cleaning or calibration required.
Verify monoclonality confidently at day zero with a DispenCell and CloneSelect Imager FL bundle
The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high throughput, automated solutions for such efforts. The general workflow below helps identify the systems that can aid in your research.
| Clone Screening System Type | Single-Cell Isolation |
| Compressed Air Description | NA |
| Minimum Operating Pressure (Imperial psi) | <.01 psi |
| Maximum Operating Pressure (Imperial psi) | NA psi |
| Optimal Operating Pressure (Imperial psi) | NA psi |
| Minimum Operating Volume Rate (Metric L/min) | NA L/min |
| Power Supply | External, PSGE120-12-01, 12v, 10A, 120W |
| Plate holder | 2 plates (96 or 384 wells) |
| Minimal cell number | 100 cells |
| Calibration | Not required |
| Cleaning | Not required |
| Sample prep. | 10 min |
| Cell parameters | Size, doublets |
| Input voltage (External power supply) | 100 - 240 V~, 50 - 60 Hz |
| Input current (External power supply) | max 2.0 A |
| Input voltage (Instrument) | 12.0 V |
| Max. power (Instrument) | 36 w |
| Well plates compatibility | "96 WP, 200µL, (655101, Greiner) 384 WP, 50µL, (781101, Greiner) 384 WP low volume, 10µL, (788096, Greiner)" |
| Loading volume | per 30s load. time @2x10^⁴ cells/mL : 10µL, 200 cells |
| Particle range detection capability | Dielectric, from 8µm to 25µm |
| Time to dispense a 96 well plate | "<= 10 min (provided Tcc < 4s) <= 13 min (provided Tcc < 6s)" |
| Time to dispense a 384 well plate | "<= 40 min (provided Tcc < 4s) <= 52 min (provided Tcc < 6s)" |
| Monoclonality reliability* | >=90% |
| Missed/Corrupted data points | < 1% per plate (only accounted for point serie n>6) |
| Objective Zoom Multiplier (X) | NA X |
| Software Description | Dispencell- driver, the operating software & Dispencell-analyzer, the analysis software installed on a PC ( NUC 10i5FNKN) without screen, mouse or keyboard ( these have to be provided by the customer) |
| Image Resolution (Metric pixels/mm) | NA pixels/mm |
| White Light Only Field of View Size (Metric mm) | NA mm |
| White Light and Fluorescence Field of View (Metric mm) | NA mm |
| Fluorescent Imaging Details | Ex /Em: 531 /593 nm for Cy3 /DS Red |
| Tracking Capability | Plate label or automated 1D barcode reader ( not compatible with special characters or spaces) |
| Bar Code Reader Count | 16 |
| Imaging Speed Description | NA |
| Imaging High Resolution (Metric μm) | NA µm |
| Imaging Standard Resolution (Metric μm) | NA µm |
| Minimum operating pressure | Less than 0.2 psi |
| Instrument weight | 11 kg |
| Instrument Size | 328 x 258.5 x 324.83 mm |