Clarity® BioSolutions
LC-MS Analysis Test
Oligo-XT
An increased need for TK and PK/PD studies for oligo therapeutics has lead to an increase in LC-MS/MS analysis because of the specificity, sensitivity, and shorter method development times compared to ligand binding assays or ELISA. Because sensitivity is of the utmost importance, it is crucial to select an LC column that provides both separation and high efficiencies to ensure accurate results.
Clarity Core-Shell
Conventional Fully Porous
The Core-Shell Advantage
Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies.
*May not be representative of all application
Clarity Oligo-XT is patented by Phenomenex. U.S. Patent No. 7,563,367 and 8,658,038 and foreign counterparts.
Analytical LC abc
Clarity Oligo-RP™
Clarity Oligo-RP columns have been specifically designed for the reversed-phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 µm particles, Clarity Oligo-RP allows for high-capacity analysis and purification of oligos.
A Highly Selective Analysis
Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.
Separation of failure N-1 from target N sequence
| Column | Clarity 3 µm Oligo-RP C18 |
|---|---|
| Dimensions | 50 x 4.6 mm |
| Part No. | 00B-4441-E0 |
| Mobile Phase |
A: 50 mM TEAA pH 7.5 B: Methanol |
| Gradient | 10 % to 45 % B in 30 minutes |
| Flow Rate | 1 mL/ min |
| Detection | UV @ 260 nm |
| Sample |
1. 40nt DNA with sequence CTTCTGAACAGTTGATCTATG- CACTTCAGACTTATGATCA (2.5 μg) 2. 39nt DNA with sequence TTCTGAACAGTTGATCTATGCAC- TTCAGACTTATGATCA (2.5 μg) |
Purification
DNA Purification
Clarity Oligo-RP columns allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.
Easily Scale from HPLC to Preparative Purification
| Column | Clarity 3 µm Oligo-RP C18 |
|---|---|
| Dimensions |
A: 50 x 10.0 mm B: 50 x 4.6 mm |
| Part No. |
A: 00B-4441-N0 B: 00B-4441-E0 |
| Mobile Phase |
A: 50 mM TEAA pH 7.5 B: Methanol |
| Gradient | 10 % to 60 % B in 20 minutes |
| Flow Rate |
A: 4.7 mL/ min B: 1.0 mL/ min |
| Detection | UV @ 260 nm |
| Sample | 20nt DNA |
