ATTO 647N
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ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region.
Optical properties
- λ abs = 646 nm
- ε max = 1.5×105 M -1 cm -1
- λ fl = 664 nm
- n fl = 65 %
- τ fl = 3.5 ns
- CF 260 = ε 260 /ε max = 0.04
- CF 280 = ε 280 / ε max = 0.03
ATTO 647N Carboxy Fluorescent Label 5 mg
Product No. AD-647N-25 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Phosphoramidite Fluorescent Label 50mg
Product No. AD-647N-3050 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Amine Fluorescent Label 5 mg
Product No. AD-647N-95 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Phosphoramidite Fluorescent Label 100mg
Product No. AD-647N-30100 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Phalloidin Fluorescent Label 10 nmol
Product No. AD-647N-81 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Alkyne Fluorescent Label 1 mg
Product No. AD-647N-141 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Amine Fluorescent Label 1 mg
Product No. AD-647N-91 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Streptavidin Fluorescent Label 1 mg
Product No. AD-647N-61 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N Cadaverine Fluorescent Label 5 mg
Product No. AD-647N-235 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N NHS-ester Fluorescent Label 1 mg
Product No. AD-647N-31 ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region.
Optical properties
- λ abs = 646 nm
- ε max = 1.5×105 M -1 cm -1
- λ fl = 664 nm
- n fl = 65 %
- τ fl = 3.5 ns
- CF 260 = ε 260 /ε max = 0.04
- CF 280 = ε 280 / ε max = 0.03
ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. Thus ATTO 647N is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
In common with most ATTO-TEC-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic. ATTO 647N is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
As supplied ATTO 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems