ATTO 550
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ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B.
Optical properties
- λ abs = 554 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 576 nm
- n fl = 80 %
- τ fl = 3.6 ns
- CF 260 = ε 260 /ε max = 0.23
- CF 280 = ε 280 / ε max = 0.10
ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B.
Optical properties
- λ abs = 554 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 576 nm
- n fl = 80 %
- τ fl = 3.6 ns
- CF 260 = ε 260 /ε max = 0.23
- CF 280 = ε 280 / ε max = 0.10
ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability.
The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence.
The fluorescence is excited most efficiently in the range 540 - 565 nm.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems