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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)(AB93684)

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Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406803

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406804

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406805

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406806

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406808

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406809

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406836

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406837

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418146

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418148

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418601

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418602

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418636

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418719

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418720

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418721

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421072

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421073

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421074

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421324

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421325

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421326

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421329

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421331

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421332

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425349

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425350

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425351

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425352

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425732

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425733

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425734

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img446063

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img446064

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img446065

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img446066

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img450020

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img450023

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img450025

Antigen retrieval buffer (100X Tris-EDTA, pH 9.0) (ab93684) is used in heat-induced antigen retrieval (HIER) to enhance staining on formalin-fixed, paraffin-embedded tissue sections with our primary antibody range during IHC.

  • Provided in 100X solution for easy dilution preparation
  • Available in different formats to fit your experimental needs. Larger volume format available upon request.
  • Compatible with our Lab essentials staining workflow offering
  • Proven performance: cited in over 85 publications

Product details - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)(AB93684)

Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH 9.0) pretreatment.

This product contains detergent for emulsification of the paraffin.

1X Dilution:

The 100X stock solution should be diluted 100-fold with distilled water before use.

Protocol:

  • Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
  • Wash sections with hot tap water for 1 minute.
  • Wash sections in buffer for 2x3 minutes.
  • Continue with IHC protocol

Other kits and reagents for IHC

Other antigen retrieval buffers include: Citrate buffer pH 6.0 ab93678, EDTA buffer pH 8.0 ab93680, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418721

Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421326

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EBI3 with ab314592 at 1/50 (10.0 ug/ml). Cytoplasmic staining on human placental trophoblasts. The section was incubated with ab314592 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img425732

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human skin (PMID : 8092987) is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img421325

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Met (c-Met) - C-terminal with ab314588 at 1/100 (5.0 ug/ml). Positive staining on human colon. The section was incubated with ab314588 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img418636

Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Antigen Retrieval Buffer 100x Tris Edta Buffer Ph 90 Ab93684 Immunohistochemistry Formalin Pfa Fixed Paraffin Embedded Sections Img406804

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 µg/ml). Cytoplasmic staining on human cerebrum. The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Specifications
Shipped At Conditions Ambient - Can Ship with Ice
Appropriate Long term Storage Conditions Store at Ambient.
Species Reactivity IHC-P