10kD Spin Column(AB93349)

The 10kD Spin Columns (ab93349) are disposable ultrafiltration devices for the concentration or deproteinization of biological samples.

They can be used in either a swing bucket or fixed angle rotors which accept 2.0 mL centrifuge tube at maximum speed 10000 x g (5-10 mins). The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area in order to achieve superior recovery from dilute solution.

Maximum initial sample volume is 500μL. The dead stop volume is approximately 20-25μL.

If working with cell or tissue lysates, we generally recommend the TCA Sample Deproteinization Kit or using a PCA/KOH Deproteinization protocol. If it is critical to use a spin column with a cell or tissue lysate then, to avoid blocking the filter, first centrifuge the lysate samples at ~14,000 rpm for 10-20 mins to pellet debris, and then use the resulting supernatant with the spin column.

Please note that filtration can be challenging with protein-rich samples like serum, as proteins may clog the filter pores. To mitigate this, use a pipette to mix the prefiltered material every few minutes during centrifugation by repeatedly drawing and expelling the liquid in the spin filter. This helps dislodge proteins accumulating on the filter.

Representative 10kD Spin Column (ab93349).