Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
ALP activity in cells undergoing osteogenic differentiation.
Hermenean A et al. (2017) measured alkaline phosphatase activity in cells cultured in CHT/GO (3D chitosan scaffolds enriched with graphene oxide) biomaterials for up to 28 days using ab83369.
Image courtesy of Hermenean A et al. Sci Rep. 2017; 7: 16641. doi: 10.1038/s41598-017-16599-5
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
Effects of mineralocorticoid receptor blockade on phosphate-induced vascular calcification in vitro.
Alesutan I et al. (2017) measured alkaline phosphatase activity in vitro in primary human aortic smooth muscle cells using ab83369. Cells were treated with phosphate (pi) or without (Ctr, and with or without additional treatment of spironolactone (Spr) or 10 μM eplerenone (Epl).
Image courtesy of Alesutan I et al. Sci Rep. 2017; 7: 2059. doi: 10.1038/s41598-017-01882-2. Reproduced under the creative commons license http://creativecommons.org/licenses/by/4.0/.
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
Alkaline phosphatase activity measured in the study of osteoporosis.
Lee YM et al. (2019) used alkaline phosphatase assay kit ab83369 to measure the serum levels of alkaline phosphatase as a marker of bone turnover. Rat groups include sham-operated (Sham), OVX-control (OVX), E2-treated OVX groups (E2), and LABE 500 mg/kg treated OVX groups (LABE) (N=10/group).
Serum ALP levels in the OVX group were significantly increased compared to those in Sham group, and oral administration of LABE (500 mg/kg) significantly reduced the serum ALP levels as an osteoporosis-related serum marker.
Image from Lee YM. et al., Biomed Res Int. 2019 Feb 19;2019:5073085. Fig 6b doi: 10.1155/2019/5073085. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
Nepelska M et al. (2012) assayed alkaline phosphatase activity in Caco-2 cells after 48 hours treatment with PMA and Butyrate using Alkaline Phosphatase assay kit (ab83369).
Image from Nepelska M et al., PLoS One 7(12), fig 8c. Doi: 10.1371/journal.pone.0052869. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
ALP measured in cell culture supernatants showing activity (U) per L of tested sample. Samples were diluted 1-16 fold.
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
ALP measured in biological fluids showing activity (U) per L of tested sample. Samples were diluted 4-64 fold.
Functional Studies - Alkaline Phosphatase Assay Kit (Colorimetric) (ab83369)
Measurement of ALP activity using ab83369. a. ALP activity in fresh medium (80 μL, without culturing), 3 day old HeLa cell culture medium (80 μL) and human serum (80 μL, 1/10 dilution). b. ALP activity in HeLa cells. 5 x 104 HeLa cells were homogenized in 1 mL of Assay Buffer, diluted 1/10 in Assay Buffer, and 80 μL used for the measurement.
