Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
ATP assay used to study mitochondrial disfunction after C. rodentium infection in mice.
Maiti AK et al (2018) used ATP assay kit ab83355 to measure mitochondrial ATP generation in an in vitro mouse intestinal model treated with cytokines in the presence and absence of VIP (vasoactive intestinal peptide). VIP was induced by C. rodentium infection and cytokines.
Image courtesy of Maiti A K et al. PLoS One. 2018; 13(9): e0204567. doi: 10.1371/journal.pone.0204567
Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
ATP levels measured in mouse colon tissue.
Maiti AK et al. (2015) used ATP assay kit ab113852 to measure mitochondrial ATP generation in murine distal colon after C. rodentium infection.
Image courtesy of Maiti A K et al. Sci Rep. 2015; 5: 1543. doi: 10.1038/srep15434. Reproduced under the Creative Commons License http://creativecommons.org/licenses/by/4.0/
Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
The chart shows a comparison of ATP levels of HepG2 treated with 0, 10 and 100 μM for 48 hours, DRH2O2 W1 (damage recovered cells using hydrogen peroxide with a recovery time of one week) HepG2 cells and MDA-MB-231 cells treated with 0, 10 and 100 μM of NaHS for 48 hours. Data is shown as percent of ATP levels in untreated cells. ATP levels were determined using ATP assay kit (ab83355).
Sanokawa-Akakura R et al., PLoS One 9(9), fig4b. doi: 10.1371/journal.pone.0108537 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
MCF-7 cells are transfected with vector, osteopontin-a, osteopontin-c or osteopontin -a plus -c. Cells are plated on poly-HEMA and seeded at 4 x 105 cells per well and incubated for two days under standard culture conditions. ATP levels are measured using ATP assay kit (ab83355).
Image from Shi Z et al., PLoS One 9(8), Fig 2 doi: 10.1371/journal.pone.0105675. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
Example of fluorometric ATP assay standard curve.
Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
Example of colorimetric ATP assay standard curve.
Functional Studies - ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)
Quantitation of ATP in fish liver (2.5μl of 10 times diluted sample), fish muscle (5μl of 10 times diluted sample) and Jurkat cell lysate (5 ul) using fluorometric assay. Samples were spiked with known amounts of ATP (300pmol).
