Anti-GFAP antibody(AB7260)

All lanes:

Western blot - Anti-GFAP antibody (ab7260) at 1/5000 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat spinal cord lysate

Lane 3:

Mouse brain lysate

Lane 4:

Mouse spinal cord lysate

Predicted band size: 49 kDa

IHC image of GFAP staining in a formalin fixed, paraffin embedded normal mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7260 at 1/2000 dilution for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.