Functional Studies - LDH Assay Kit (Cytotoxicity) (ab65393)

Bafilomycin A1 (BafA1) toxicity was assessed in Human hepatic (Huh7.5) cells after 24 hours at different concentrations (12.5nm, 25nm, 50nm and 100nm) were administered to cells using LDH cytotoxicity assay kit (ab65393). Cytotoxicity was measured by subtracting LDH content in remaining viable cells from total LDH in untreated controls.

Image from Bukong TN et al., PLoS Pathog 10(10), Fig S6. Doi: 10.1371/journal.ppat.1004424. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - LDH Assay Kit (Cytotoxicity) (ab65393)

Jurkat T cells were cultured in 96-well plate in 100 μl of culture medium. LDH Assay was performed using 10μl of culture medium using the WST probe. Low control (white bar); High control (black bar).

Functional Studies - LDH Assay Kit (Cytotoxicity) (ab65393)

Comparison of WST-1 and INT based LDH assays. 3T3 cells were cultured in a 96-well plate in 100 μl of culture medium. The LDH assay was performed using 10 μl of culture medium using WST-1 (Brown bar) and INT (Green bar) methods. The WST-1 based LDH assay is more stable and sensitive than the INT based method.