Functional Studies - Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359)
Assessment of free and total cholesterol and cholesteryl ester levels useing ab65359. Protein levels of geranylgeranyldiphosphate synthase were measured by ELISA. Absolute amounts of CoQ10 were measured by HPLC as described in Materials and Methods. Cholesterol levels were quantified using a Cholesterol/Cholesteryl Ester Detection Kit from Abcam according to instruction. n?=?6, means ±SEM. Two-way ANOVA with Tukey's post-hoc test. * denotes statistical comparison between vehicle control (V) and high dose (H) and # denotes statistical comparison between water and ethanol treated groups. * and **, indicate significant difference at p<0.05, 0.01 and 0.0001, respectively. OWV; obese water vehicle, OWH; obese water high dose, OEV; obese ethanol vehicle, OEH; obese ethanol high dose.
Mailloux RJ, Florian M, Chen Q, Yan J, Petrov I, Coughlan MC, et al., PLoS ONE 9(9): e106832., Fig 3, doi.org/10.1371/journal.pone.0106832
Functional Studies - Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359)
Total cholesterol (free and ester forms) were determined with a cholesterol quantification assay (fluorometric detection) (ab65359) and are shown relative to untreated control cells.
Singh, Natesh and Gerhard F Ecker., International journal of molecular sciences vol. 19,5 1278., Fig 1, doi:10.3390/ijms19051278
Functional Studies - Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359)
Cholesterol and cholesteryl ester were quantified colourimetrically in biological fluids (dilution range 1:10-1:100; 50 minutes incubation) and measured in duplicates (+/- SD).
Functional Studies - Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359)
Detection of Cholesterol/Cholesteryl Ester Using Cholesterol Quantitation Kit (ab65359). Cholesterol/Cholesteryl Ester was quantified using the kit by colorimetric (A) and fluorometric (B) methods according to the kit instructions. Background from the control reaction (without cholesterol) has been subtracted from each value. Note: Fluorometric assay is over 10 fold more sensitive than colorimetric assay.
