Functional Studies - NAD/NADH Assay Kit (Colorimetric) (ab65348)

NADH/NAD+ ratio in soleus muscle measured useing ab65348. p ? 0.05 when comparing (+) HFD and LFD, (*) HFD + TQ and HFD, and (#) LFD and LFD + TQ using independent t-tests. Results are means ± SEM (n = 8-10 mice per treatment group). LFD: low fat diet, HFD: high fat diet, TQ: thymoquinone.

Karandrea, Shpetim et al., PloS one vol. 12,9 e0185374., Fig 6, doi:10.1371/journal.pone.0185374

Functional Studies - NAD/NADH Assay Kit (Colorimetric) (ab65348)

NAD/NADH was measured in K562 ME2 knockdown cells(pLKO - empty vector; shME2-2 & shME2-3 - two selected knockdown clones). Data are expressed as mean ±: SD, n=3. NAD/NADH Ratio is calculated as described in the product protocol.

Image obtained from Ren JG et al; PLOS one, 2010; 5(9): e12520 (DOI:10.1371/journal.pone.0012520)

Ren JG et al., PLoS One, 5, , 2010 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - NAD/NADH Assay Kit (Colorimetric) (ab65348)

NAD and NADH (tNAD) or NADH alone measured cell lysates. 5e6 cells were lysed in 1 mL, spin filtered, and tested neat or 1/5 (duplicates +/- SD).

Functional Studies - NAD/NADH Assay Kit (Colorimetric) (ab65348)

Standard curve with background signal subtracted (duplicates; +/- SD).

Functional Studies - NAD/NADH Assay Kit (Colorimetric) (ab65348)

NADH Standard calibration curve. Quantification of NAD (diamond) and NADH (open square) following product protocol and using NADH standard provided in the kit. No NADP (triangle) was detected in this reaction. Standard curve range: 20-100 pmol.