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Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)

Lipolysis were evaluated by measuring free fatty acids released into the culture medium of siCreg1 or si-Scramble 3T3-L1 cells using ab65341, which were treated with BMS-345541 (5 μmol/L), an NF-κB pathway inhibitor, or vehicle (DMSO) for 48 hours prior to lipolysis assessment. ns, no significance; and ***P<0.001.

Tian, Xiaoxiang et al., PloS one vol. 12,5 e0176873., Fig 6, doi:10.1371/journal.pone.0176873

Free fatty acid assay kit (ab65341) used to evaluate lipolysis in 3T3-L1 cells treated with NF-κB inhibitor BMS-345541 or vehicle for 48 hours.

Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)

Plasma FFA levels were measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).

Samuel P et al., PLoS One 8(1), Fig 7a. DOI: 10.1371/journal.pone.0048425. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Plasma FFA levels measured using ab65341 in wild-type and AT2KO mice on normal or high-fat diet, as part of Free Fatty Acid Assay Kit study.

Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)

Func S-1.

Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

Functional studies of free fatty acid assay kit (ab65341) showing colorimetric standard curve with mean of duplicates and background subtraction

Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)

Free Fatty Acid measured in biologicals showing concentration (μM).

Free fatty acid assay kit (ab65341) for quantifying free fatty acids in biological samples with concentration in μm

Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)

Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

Fluorometric standard curve for free fatty acid assay kit (ab65341) showing mean of duplicates with background readings subtracted.
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