L-Lactate Assay Kit (Colorimetric/Fluorometric)(AB65330)
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Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
HaCaT cells are grown in normoxia for 3 days after infection and medium was tested to determine lactate concentration using L-Lactate assay kit (ab65330). HaCaT cells were infected with AdGFP-18E2 or AdGFP, transfected with Ctrl or HIF-1α siRNA.
Image from Lai D., PLiS One 8(9), Fig 6C. Doi: 10.1371/journal.pone.0075625. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Lactate measured in cell culture supernatants showing quantity (μmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Lactate measured in biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 10-40 fold and measured fluorometrically.
| Shipped At Conditions | Blue Ice |
| Appropriate Long term Storage Conditions | Store at -20°C. |
| Clonality | Polyclonal |
| Applications | ICC/IF, IHC-P, WB |
| Species Reactivity | Human |
| Isotype | IgG |