Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)

HaCaT cells are grown in normoxia for 3 days after infection and medium was tested to determine lactate concentration using L-Lactate assay kit (ab65330). HaCaT cells were infected with AdGFP-18E2 or AdGFP, transfected with Ctrl or HIF-1α siRNA.

Image from Lai D., PLiS One 8(9), Fig 6C. Doi: 10.1371/journal.pone.0075625. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)

Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).

Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)

Standard curve: mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)

Standard curve: mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)

Lactate measured in cell culture supernatants showing quantity (μmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.

Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)

Lactate measured in biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 10-40 fold and measured fluorometrically.