Anti-α-Synuclein (phospho S129) Antibody (ab51253)

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

Expression of WT aSyn in the SN is toxic over time. Mouse brain sections immunostained for TH (red panels) and aSyn (using ab51253) (green panels) 1, 2 and 3 weeks after injection with vectors encoding for EGFP or WT aSyn. Scale bar for isolated channels 200 μm and for merged channels 100 μm.

This image is taken from Machado de Oliveira. R et al. PLoS Biol. 2017 Mar; 15(3): e2000374. S7a doi: 10.1371/journal.pbio.2000374 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

Microscopic image of mouse brain sections stained for proteins: red shows Tyrosine Hydroxylase (TH), green shows alpha-synuclein (phospho S129).

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

α‐Synuclein (α‐syn) and S129‐phosphorylated α‐synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α‐synuclein (using ab1903) and serine 129 (S129)‐phosphorylated α‐synuclein protein (using ab51253) in the brainstem (example blots shown).

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

Predicted band size: 14 kDa

This image is taken from Migdalska-Richards A. et al. Ann Neurol. 2016 Nov; 80(5): 766–775. Fig 5 doi: 10.1002/ana.24790

Western blot analysis of α-synuclein and S129-phosphorylated α-synuclein protein levels in SNCA/SNCA mouse brains treated with ambroxol.

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

Blocking and Diluting buffer and concentration - 5% NFDM/TBST

Exposure time: 3 seconds

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of AT

Lane 2:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2

Lane 3:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa