Western blot - Anti-Iba1 antibody (ab5076)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Iba1 antibody (ab5076) at 1 µg/mL

Lane 1:

Human lymph node tissue lysate in RIPA buffer at 35 µg

Lane 2:

Rat brain tissue lysate in RIPA buffer at 35 µg

Developed using the ECL technique.

Predicted band size: 16 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lateral prefrontal cortex tissue labeling iba1 labeled microglia (labeled with black arrows) within the cortex using ab5076 at 1/1000 dilution. (Cropped image).

Sections were rinsed in 0.01 m PBS, pH 7.4, followed by 10% serum matching the species of the secondary antibody, 5% bovine serum albumin, and 0.1% Triton X-100 in 0.01 m PBS blocking solution for 1 h and incubated for 2 days at 4 °C in primary antibody. The sections were rinsed in PBS and incubated overnight at 4 °C with a horse anti-Goat IgG (Biotinylated) secondary antibody.

Image from Iris Margalit Trutzer et al, ACTA neuropathologica communications, 7, 40, Fig. 7; doi: 10.1186/s40478-019-0684-8

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with ab5076 at 8µg/ml.

Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)

Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Iba1 with no primary antibody.

Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.