Western blot - Anti-Collagen I antibody (ab34710)

DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.

Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

Other Band(s): Collagen Type I splice variants and isoforms.

All lanes:

Western blot - Anti-Collagen I antibody (ab34710) at 1/1000 dilution

All lanes:

Western blot - Native Human Collagen I protein (ab7533) at 0.05 µg

Secondary

All lanes:

DyLight™ 649 anti-rabbit secondary antibody at 1/20000 dilution

Predicted band size: 139 kDa