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Immunoprecipitation - Anti-STAT6 antibody [YE361] (ab32520)

ab32520 (purified) at 1/20 immunoprecipitating STAT6 in 10 μg NIH/3T3 (mouse embyro fibroblast cell line; Lanes 1 and 2, observed at 100 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-STAT6 antibody [YE361] (ab32520)

Predicted band size: 94 kDa

Western blot of STAT6 immunoprecipitation in NIH/3T3 cells

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

Immunohistochemical staining of paraffin embedded human kidney with purified ab32520 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of human kidney tissue showing antigen expression with hematoxylin counterstain and negative control inset.

Western blot - Anti-STAT6 antibody [YE361] (ab32520)

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM/TBST

All lanes:

Western blot - Anti-STAT6 antibody [YE361] (ab32520) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 10 µg

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa

Western blot analysis of STAT6 expression in NIH/3T3 and RAW 264.7 cells

Western blot - Anti-STAT6 antibody [YE361] (ab32520)

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM/TBST

All lanes:

Western blot - Anti-STAT6 antibody [YE361] (ab32520) at 1/20000 dilution

All lanes:

Raji (human Burkitt's lymphoma cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa

Western blot of STAT6 protein in Raji cell lysate

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

Mouse kidney tissue stained with anti-STAT6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling STAT6 with unpurified ab32520 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

STAT6 expression in human skin carcinoma tissue
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