Sulfo-LC-SPDP(ab285422)

MW 527.6 Da, Purity >=90%. Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed. Amine-reactive; the long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 AÌŠ spacer arm. More water-soluble than the corresponding LC-SPDP. Membrane-impermeable which enables crosslinking on cell surface. The cleavable spacer arm enables separation of cross-linked products. APPLICATIONS: Reversible crosslinking of proteins. N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains. Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.