Mouse ALT ELISA Kit(AB282882)

Example of mouse ALT standard curve in 1X Cell Extraction Buffer PTR.

The ALT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Interpolated concentrations of native ALT in mouse serum, plasma samples.

The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma – citrate 5%, plasma -heparin 5% and plasma – EDTA 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 67.60 ng/mL in serum, 30.36 ng/mL in plasma – citrate, 48.30 ng/mL in plasma – heparin and 38.86 ng/mL in plasma - EDTA.

Interpolated concentration of native ALT was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Example of mouse ALT standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.