Human Erythropoietin ELISA Kit (EPO)(ab274397)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)

Example of human Erythropoietin standard curve in Sample Diluent NS.

The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)

Interpolated concentrations of native Erythropoietin in human serum, plasma and HepG2 cell culture supernatant samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, Plasma (EDTA) 100%, plasma (heparin) 100% and Hep G2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 16 mIU/mL in neat serum, 24 mIU/mL in neat plasma (citrate), 20 mIU/mL in neat plasma (EDTA), 20 mIU/mL in neat plasma (heparin) and 66 mIU/mL in neat Hep G2 supernatant.

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 23.4 mIU/mL with a range of 3.3 – 75.7 mIU/mL.