Human LDLR ELISA Kit(AB270212)

Example of human LDLR standard curve in Sample Diluent NS. as described in Section 10.

The LDLR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Interpolated concentrations of native LDLR in human serum and plasma samples.

The concentrations of LDLR were measured in duplicates, interpolated from the LDLR standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 34.6 ng/mL in serum, 41.6 ng/mL in plasma (citrate) and 24.9 ng/mL in plasma (EDTA), and 31.4 ng/mL in plasma (heparin).

Interpolated concentrations of native LDLR in human urine and cell culture supernatant samples.

The concentrations of LDLR were measured in duplicates, interpolated from the LDLR standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 2% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 223.5 ng/mL in urine and 28.0 ng/mL in HepG2 cell culture supernatant.

Interpolated concentrations of native LDLR in HepG2 cell extract based on a 200 μg/mL extract load.

The concentrations of LDLR were measured in duplicate and interpolated from the LDLR standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 11.8 ng/mL in sample.

Serum from nine individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LDLR concentration was determined to be 65 ng/mL with a range of 38 – 115 ng/mL.