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Cell Counting Kit 8 (WST-8 / CCK8)(AB228554)

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Cell Counting Kit 8 (WST-8 / CCK8)(AB228554)

Cell Counting Kit 8 (WST-8 / CCK8)(AB228554)

Cell Counting Kit 8 ab228554 is a no-wash assay with one incubation of 1-4 hours. WST-8 / CCK8 tetrazolium in solution is very stable with low cytotoxicity, and is reduced by cellular dehydrogenases with colorimetric (460 nm) readout proportional to the number of living cells.

Cell Counting Kit designed for success:
- Simple and fast protocol with ready to use solution
- Higher sensitivity and stability than other tetrazolium salt-based assays MTT, MTS or XTT
- Highly cited in >200 publications

Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)

Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with The cell Counting Kit 8 (WST-8) (ab228554). HeLa cells at 10,000 cells/well/100 μL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a plate reader.

Functional Studies - Cell Counting Kit 8 (WST-8 / CCK8) (ab228554)

Cell Counting Assay.

Cell number was determined with the cell Counting Kit 8 (WST-8) (ab228554). (A) HeLa cells at 0 to 10,000 cells/well/100 μL, and (B) Jurkat cells at 0 to 100,000 cells/well/100 μL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a plate reader.

Specifications
Shipped At Conditions Blue Ice
Appropriate Long term Storage Conditions Store at +4°C.
CAS Number 193149-74-5