Mouse Progranulin ELISA Kit(AB213473)

Example of mouse Progranulin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Example of mouse Progranulin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Interpolated concentrations of native Progranulin in mouse serum and plasma samples.

The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:250, plasma (citrate) 1:250, plasma (EDTA) 1:250, and plasma (heparin) 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 1,610.5 ng/mL in serum, 1,149.5 ng/mL in plasma (citrate), 1,253.7 ng/mL in plasma (EDTA) and 1,710.8 ng/mL in plasma (heparin).

Interpolated concentrations of native Progranulin in mouse J774A.1 and RAW264.7 cultured media samples and spiked Progranulin in RPMI culture media.

The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows: J774A.1 cultured media 1:400, RAW264.7 cultured media 6.25%, and RPMI culture media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 197.4 ng/mL in J774A.1 cultured media, 38.4 ng/mL in RAW264.7 cultured media, and 82.6 ng/mL in RPMI culture media. J774A.1 samples were cultured in high glucose DMEM culture media with kanamycin and 10% fetal bovine serum for 72 hours and then stimulated with 1.5% PHA plus 10 ng/mL PMA for 72 hours. RAW264.7 samples were cultured in high glucose DMEM culture media with kanamycin and L‑Glutamine for 72 hours and then stimulated with 5 μg/mL of LPS for 48 hours.

Interpolated concentrations of native Progranulin in mouse J774A.1 cell extract based on a 12.5 μg/mL extract load.

The concentrations of Progranulin were measured in duplicate and interpolated from the Progranulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration in J774A.1 cell extract was determined to be 2.9 ng/mL.

Interpolated concentrations of native Progranulin in mouse J774A.1 cultured media samples.

All samples were diluted 1:400 and the concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 34.8 ng/mL in unstimulated J774A.1 cultured media and 206.9 ng/mL in stimulated J774A.1 cultured media. J774A.1 samples were cultured in high glucose DMEM culture media with kanamycin and 10% fetal bovine serum for 72 hours (unstimulated samples) and then stimulated with 1.5% PHA plus 10 ng/mL PMA for 72 hours.