Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)
Example of mouse TNF alpha standard curve in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)
Example of mouse TNF alpha standard curve in Sample Diluent 25BS.
The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)
Example of mouse TNF alpha standard curve in Sample Diluent 10BS.
The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)
Example of mouse TNF alpha standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)
Example of mouse TNF alpha standard curve in Sample Diluent NS.
The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)
Interpolated concentrations of spiked TNF alpha in mouse plasma samples.
The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 1.68 ng/mL in plasma (citrate), 1.81 ng/mL in plasma (EDTA) and 1.47 ng/mL in plasma (heparin).
