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Mouse TNF alpha ELISA Kit(AB208348)

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Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img106127

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img106126

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img99251

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img51620

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img136455

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img36489

Mouse TNF alpha ELISA Kit ab208348 is a single-wash 90-min SimpleStep ELISA used to quantify Mouse TNF alpha with a sensitivity of 9.1 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Mouse TNF alpha sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img51620

Example of mouse TNF alpha standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img106126

Example of mouse TNF alpha standard curve in Sample Diluent 25BS.

The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img106127

Example of mouse TNF alpha standard curve in Sample Diluent 10BS.

The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img136455

Example of mouse TNF alpha standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img99251

Example of mouse TNF alpha standard curve in Sample Diluent NS.

The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab208348)

Mouse Tnf Alpha Elisa Kit Ab208348 Sandwich Elisa Img36489

Interpolated concentrations of spiked TNF alpha in mouse plasma samples.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 1.68 ng/mL in plasma (citrate), 1.81 ng/mL in plasma (EDTA) and 1.47 ng/mL in plasma (heparin).

Specifications
Shipped At Conditions Blue Ice
Appropriate Long term Storage Conditions Store at +4°C.
Applications sELISA