TUNEL Assay Kit - HRP-DAB(AB206386)

TUNEL assay staining.

Gao Y et al used In situ Apoptosis Detection Kit / TUNEL assay ab206386 to analyze tissue sections from mouse ovaries.

a. Section treated with DNase I as positive control

b. Negative control without TdT enzyme

c and f. representative experimental images.

Nuclei stained with the TUNEL assay are brown. Sections were counter-stained with Methyl Green.

Gao Y et al. Reproductive Biology and Endocrinology 15:94 (2017) Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Using paraffin fixed human tonsil tissue,10 μm sections (1000X). A] Section processed and counter-stained with methyl green according to the manual. B] Counter-stain step was eliminated to more clearly illustrate the level of positive staining in the germinal centres of tonsil tissue. C] Section treated with DNase I in order to generate a positive control slide. Note all nuclei stain positive. The use of DNase I generates free 3'-OH groups on cellular DNA, these free 3'-OH groups are then labelled with biotin-nucleotide by the TdT in the kit. D] Negative control, the TdT enzyme step was eliminated thereby generating a negative slide.

Using paraffin fixed human tonsil tissue, 10 μm sections (1000X)