Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796)
Parkash, Om, et al used Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796) as part of the development and evaluation of a biosensor based on screen-printed carbon electrodes (SPCEs) for the detection of dengue-specific immunoglobulin M (IgM) antibodies. They used the kit to conjugate Biotinylation Kit / Biotin to anti-Human IgM antibody for use in conjugation.
(A) Schematic diagram of the screen printed carbon electrode (SCPE)-based dengue IgM biosensor. The biosensor was constructed by sequentially adding optimised concentration of anti-human IgM capture antibody, blocking agent, human IgM antibody or serum sample, dengue antigen and detection antibody, with washing steps in between. Electrochemical signal was generated following addition of TMB substrate. (B) Comparison of various immobilisation techniques for the goat anti-human IgM capture antibody. NC: negative control consisting of a dengue IgM negative serum sample; PC: dengue IgM positive serum sample. (C) Field Emission Scanning Electron Microscopy (FESEM) surface images of (left panel) a bare carbon electrode and (right panel) a carbon electrode modified with an anti-human IgM antibody using the streptavidin/biotin immobilisation system. Both capture and detection antibodies were labeled using Lightning-Link® conjugation kits (ab201796 and ab102890).
Image from Parkash, Om, et al., Diagnostics (Basel), 11(1):33; doi: 10.3390/diagnostics11010033. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (ab201796)
Rackus, Darius G., et al used Biotinylation Kit / Biotin Conjugation Kit (Type B) - Lightning-Link® (ab201796) as part of examining digital microfluidics for detection a malaria biomarker. They used the kit to conjugate Biotinylation Kit / Biotin to anti-Plasmodium falciparum LDH antibody for use in pre-concentration by liquid intake by paper (P-CLIP).
DMF immunoassay for PfLDH. Comparison of mean signals obtained by traditional DMF-ELISA (black) and P-CLIP modified DMF-ELISA (grey) for concentrations below the limit of detection (7 ng mL-1) and limit of quantitation (70 ng mL-1) of the traditional DMF-ELISA. Error bars ± 1 std. dev. (n = 3).
Image from Rackus et al., Lab Chip., 17(13):2272-2280; doi: 10.1039/c7lc00440k. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/3.0/
