Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Mouse IL-1 beta standard curve comparison data.
Standard curve comparison between mouse IL-1 beta SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of IL-1 beta standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.
RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 μg/mL extract load.
The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 70 pg/mL in LPS stimulated RAW 264.7 Lysate.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
