Immunocytochemistry - Anti-c-Fos antibody - BSA free (ab190289)
Immunocytochemistry analysis of HeLa cells labeling c-Fos with ab190289 at 1/5000 dilution (red) and mouse mAb to tubulin at 1/10,000 dilution (green). The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) – free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells.
Western blot - Anti-c-Fos antibody - BSA free (ab190289)
Mouse Monoclonal Antibody to GAPDH, dilution 1/5000, was used as a loading control (red).
Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein.
All lanes:
Western blot - Anti-c-Fos antibody - BSA free (ab190289) at 1/2000 dilution
Lane 1:
Protein standard
Lane 2:
HeLa cells grown in FBS free media
Lane 3:
HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours
Lane 4:
Rat cortical neurons
Lane 5:
Rat cortical neurons treated with membrane depolarization buffer for 5 hours
Predicted band size: 41 kDa
Immunohistochemistry - Free Floating - Anti-c-Fos antibody - BSA free (ab190289)
Immunohistochemistry - Free Floating analysis of mouse hippocampus (C) or olfactory bulb sections (D) labeling c-Fos wiht ab190289 at 1/20,000 (red) and mouse mAb to NF-L at 1/5,000 (green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.
Immunocytochemistry - Anti-c-Fos antibody - BSA free (ab190289)
Immunofluorescent analysis of HeLa cells (serum–starved, 36 hours followed by 20% FBS treatment for 2 hours) labeling c-Fos with ab190289 at 1/2000 dilution (red). Cells were counterstained with chicken polyclonal antibody against vimentin (green) and DAPI (blue).
Western blot - Anti-c-Fos antibody - BSA free (ab190289)
All lanes:
Western blot - Anti-c-Fos antibody - BSA free (ab190289) at 1/2000 dilution
Lane 1:
HeLa cell lysate from cells serum-starved for 36 hours
Lane 2:
HeLa cell lysate from cells serum-starved for 36 hours then stimulated with 20% fetal bovine serum for 2 hours
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody - BSA free (ab190289)
Immunohistochemistry analysis of mouse brain sections across hippocampus labeling c-Fos with ab190289 at 1/2000 dilution (red). Cells were counterstained with anti Fox3/NeuN antibody (green) and DAPI (blue). Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of c-Fos staining.
