Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

All lanes:

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/10000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

Immunohistochemistry (Frozen sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

Immunohistochemistry (Frozen sections) analysis of rat small intestine tissue sections labeling TOMM20 with Purified ab186735 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

ab186735 staining TOMM20 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control: PBS only

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

All lanes:

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/2000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg

Lane 2:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)

Intracellular Flow Cytometry analysis of TOMM20 expression in paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells cells using ab186735 at 1/90 dilution (red) and a rabbit IgG as negative control (green).