Anti-SOX9 antibody [EPR14335-78](AB185966)

ab185966 staining Sox9 in F9 (Mouse embryonic testicular cancer cell line) cells.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab185966 at a 5 μg/ml concentration and ab7291, mouse monoclonal [DM1A] to alpha Tubulin, at 1μg/ml concentration, followed by a further incubation at room temperature for 1 h with an anti-rabbit AlexaFluor® 488 (ab150081) at 2 μg/ml (shown in green) and an anti-mouse AlexaFluor® 594 (ab150120) at 2 μg/ml (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling SOX9 with purified ab185966 at 1/120 (red).

Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry analysis of paraffin-embedded rat colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.

Immunohistochemistry analysis of paraffin-embedded mouse colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.

Immunohistochemistry analysis of paraffin-embedded human colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.