Anti-CD163 antibody [EPR19518](AB182422)
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- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with Rb1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR17512 is cited in over 90 publications
New 20 ul size available
Flow Cytometry - Anti-CD163 antibody [EPR19518] (ab182422)
Flow cytometry analysis of human PBMC cells (Human peripheral blood mononuclear cell) labeling with ab182422 at 1/60 dilution, 11.23 μg/ml (red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody at 1/2000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] (ab182422)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse spleen is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
| Shipped At Conditions | Blue Ice |
| Appropriate Long term Storage Conditions | Store at -20°C. |
| Clonality | Monoclonal |
| Applications | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB |
| Species Reactivity | African green monkey, Human, Mouse |
| Isotype | IgG |