Human Albumin ELISA Kit(AB179887)

Human Albumin standard curve comparison.

Standard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.

Background-subtracted data values (mean +/- SD) are graphed.

Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.

The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.

Linearity of dilution for spiked albumin.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Spiked Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Linearity of dilution for albumin.

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.