Human IL-6 ELISA Kit(AB178013)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Example of Human IL-6 standard curve in Sample Diluent NS in 96-well and 384-well plate.

Example of Human IL-6 standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Interpolated concentrations of Human IL-6 in human stimulated PBMC supernatant in 96-well and 384-well plates.

Interpolated concentration of native IL-6 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2% Human Stimulated PBMC supernatant treated with 1.5% PHA-M for 46 hrs. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Example of human IL6 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (ab178013). Wild-type and IL-6 knockout A549 cells (ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.