Human IL-6 ELISA Kit (ab178013) | Danaher Life Sciences

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Human IL6 standard curve comparison data.

Standard curve comparison between the previous Human IL6 SimpleStep ELISA® kit and the re-developed Human IL6 SimpleStep ELISA® kit. The re-developed kit shows increased sensitivity.

Human IL6 standard curve comparison showing increased sensitivity in re-developed kit.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Example of human IL6 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Human IL6 standard curve with background-subtracted data values, showing mean +/- SD.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Example of raw data value for of human IL-6 standard curve.

Raw data values are shown in the table.

Raw data values for human IL-6 standard curve.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Interpolated concentrations of spiked IL6 in human serum and plasma samples.

The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Interpolated concentrations of spiked IL6 in serum and plasma samples, corrected for dilution, showing mean +/- SD.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Interpolated concentrations of native IL6 in human peripheral blood monocyte (PBMC) cell culture supernatant samples.

The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell culture supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL6 concentration was determined to be 60,443 pg/mL in stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.

Interpolated concentrations of native IL6 in PBMC cell culture supernatant, corrected for dilution, showing mean IL6 levels.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Linearity of dilution for spiked recombinant IL-6.

Recombinant IL-6 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent NS. NL= Non linear 50% pooled serum and plasma (EDTA, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

50% serum from ten individual healthy human male and female donors was measured in duplicate. All values were below the detectable range of the assay.

Linearity of dilution for spiked recombinant IL-6 in biological samples, measured in duplicate.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Linearity of dilution for native IL-6.

Native IL-6 was measured in biological samples in a 2- fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Interpolated concentrations of Human IL-6 in human stimulated PBMC supernatant in 96-well and 384-well plates.

Interpolated concentration of native IL-6 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2% Human Stimulated PBMC supernatant treated with 1.5% PHA-M for 46 hrs. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Example of Human IL-6 standard curve in Sample Diluent NS in 96-well and 384-well plate.

Example of Human IL-6 standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (ab178013). Wild-type and IL-6 knockout A549 cells (ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.