ELISA - Human Frataxin ELISA Kit (ab176112)
Titration of HeLa cell extract within the working range of the assay.
Background subtracted data from duplicate measurements are plotted.
ELISA - Human Frataxin ELISA Kit (ab176112)
Quantification of Frataxin expression in different cell lines.
Interpolated values of Frataxin are plotted for the indicated cell lines based on a extract load of 10 μg/mL.
Sandwich ELISA - Human Frataxin ELISA Kit (ab176112)
Linearity of dilution for Recombinant Frataxin.
Recombinant Frataxin was measured in biological samples in a 2-fold dilution series
Sandwich ELISA - Human Frataxin ELISA Kit (ab176112)
Raw data value for example of frataxin standard curve.
Raw data values are shown in the table
Sandwich ELISA - Human Frataxin ELISA Kit (ab176112)
Example of Frataxin standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Frataxin ELISA Kit (ab176112)
Quantification of Frataxin expression in different human patient samples.
Transformed B lymphocyte cells from Friedreich's Ataxia (FRDA) samples, due to a stable homozygous GAA repeat insertion, are compared to heterozygous carrier B lymphocyte cells and control B lymphocyte cells. B lymphocyte cell extracts were analyzed across a 7-point titration (0.1-100 μg/mL) and frataxin levels were interpolated from the standard curve. Average interpolated values of Frataxin are plotted.
