Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)

Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.

Western blot - Anti-mCherry antibody (ab167453)

All lanes:

Western blot - Anti-mCherry antibody (ab167453) at 1/1000 dilution

Lane 1:

HEK293 cells transfected with pFin- EF1-mCherry vector

Lane 2:

Non-transfected HEK293 cells