Anti-ErbB2 / HER2 antibody [SP3](AB16662)

Formalin-fixed, paraffin-embedded human breast invasive ductal carcinoma tissue stained for ErbB2 / HER2 using ab16662 at 1/200 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG HRP. Heat mediated antigen retrieval was done using a pH 8.5 buffer.

This image was generated from the hybridoma version.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB 2 with ab16662, followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on Human breast carcinoma, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab16662 for 10 mins at room temperature. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10 minutes. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

This image was generated from the hybridoma version.

Immunohistochemical analysis of formalin fixed paraffin embedded human breast cancer labelling ErbB2 with ab16662 at a concentration of 0.16 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab16662 anti-ErbB2 / HER2 antibody [SP3] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.