Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (ab15580)
Confocal images of mouse trachea epithelium collected at steady state, 24 and 48 h after SO2 injury. Tissue sections were co-stained with UHRF1 and Ki67, a proliferation marker.
ab15580 was used to stain Ki67 at a dilution of 1:1 000
Image courtesy of Xiang H. et al. Cell Discov. 2017; 3: 17019. doi: 10.1038/celldisc.2017.19 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
Western blot - Anti-Ki67 antibody (ab15580)
Observed band sizes : 345kDa, 395kDa
All lanes: Western blot - Anti-Ki67 antibody (ab15580) at 1 µg/mL
All lanes: Hela whole cell lysate at 20 µg
Secondary
All lanes: Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody (ab15580)
ab15580 staining Ki67 in Mef1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab15580 at 0.5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody (ab15580)
ab15580 staining Ki67 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab15580 at 0.5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
