Fluo-8 AM, green fluorescent calcium binding dye(AB142773)

MW 1046.94 g/mol. Medium affinity green fluorescent calcium binding dye. Binds to intracellular calcium (Kd = 390 nM). Fluorescence intensity increases upon Ca2+ binding. Cell-permeable. Fluo-8 (or Fluo-2 Medium Affinity) has been found to be brighter (1.5x) than Fluo-4 in cellular experiments. It offers improved cell loading and Ca2+ response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at 490 nm and maximum emission at 520 nm. Fluo-8 loading can be performed at room temperature.