PKC Kinase Activity Assay Kit(AB139437)

1.5 x e7 THP-1 cells were incubated with 100 nM GF109203X (GF; ab144264) or Ro31-8220 mesylate (Ro; ab120374) for 30 minutes prior to activation with 10 μg x mL-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 μL were tested for PKC activity (duplicates; +/- SD).

 

Signal from active PKC with background signal subtracted (duplicates; +/- SD).

Titration of ab139437 (duplicates; +/- SD).

schematic diagram of the assay.

Fitzpatrick, Daniel P. (2020) "A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide," : Vol. 2 : Iss. 1, Article 2.

Available at : https : //arrow.tudublin.ie/sure_j/vol2/iss1/2

This image is courtesy of Fitzpatrick, Daniel P. (2020) "A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide," Sure-J: Science Undergraduate Research Journal: Vol. 2: Iss. 1, Article 2

REACH authorisation

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

Intra assay

Sample	n	C.V.
Sample	0	< 10

Inter assay

Sample	n	C.V.
Sample	0	< 10

What's included?

1 x 96 Tests

Components	Unit & Qty
20X Wash Buffer	1 x 30 mL
Active PKC	1 x 30 µL
Anti-Rabbit IgG: HRP Conjugate	1 x 20 µL
Antibody Dilution Buffer	1 x 10 mL
ATP	1 x 2 mg
Kinase Assay Dilution Buffer	1 x 10 mL
PKC Phosphospecific Substrate Antibody	1 x 5 mL
PKC Substrate Microtiter Plate	1 x 1 Unit
Stop Solution 2	1 x 10 mL
TMB Substrate	1 x 10 mL

Properties and storage information

Shipped at conditions	Dry Ice
Appropriate short-term storage conditions	Multi
Appropriate long-term storage conditions	Multi
Storage information	Please refer to protocols

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.
Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli. 

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.