Anti-Somatostatin Receptor 2 Antibody (ab134152)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Immunohistochemical analysis of paraffin-embedded human cervical cancer xenograft tissue (cells derived from liver metastases) using ab134152 at 1/100 dilution.

Somatostatin Receptor 2 is detected using ab134152.

From Figure 1 of Yuan et al.

Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617, PMID: 28378747

Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/

Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617

Immunohistochemical analysis of paraffin-embedded human cervical cancer xenograft tissue (cells derived from liver metastases) using ab134152 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Blocking and diluting buffer: 5% NFDM/TBST.

This antibody doesn't work in MCF-7 and MDA-MB-231 cells which should be positive (PMID: 16519802). A549 cell is Somatostatin Receptor 2 negative cell line (PMID: 19148511).

All lanes: Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

Lane 1: U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 2: U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 3: MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 4: MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 5: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 6: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 7: A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Secondary

All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 73 kDa

Exposure time: 3min

Western blot detecting Somatostatin Receptor 2 with anti-Somatostatin receptor 2 antibody [UMB1] (ab134152) at 1/1000 dilution. Ineffective in MCF-7 and MDA-MB-231 cells; A549 cells are negative.

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: Lane 1 and 2: 1 second. Lane 3 and 4: 5 seconds.

All lanes: Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

Lane 1: Human brain lysates prepared in RIPA lysis method at 15 µg

Lane 2: Human brain lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 3: Mouse brain lysates prepared in RIPA lysis method at 15 µg

Lane 4: Mouse brain lysates prepared in 1% SDS hot lysis method at 15 µg

Secondary

All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 73 kDa

Western blot using anti-Somatostatin receptor 2 antibody [UMB1] (ab134152) at 1/1000 dilution with 5% NFDM/TBST. Exposure times: 1 second for lanes 1 and 2, 5 seconds for lanes 3 and 4

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

All lanes: Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

Lane 1: U937 (Human histiocytic lymphoma cell line) cell lysates at 10 µg

Lane 2: INS-1 cell lysates at 10 µg

Lane 3: Somatostatin Receptor 2 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysates at 10 µg

Secondary

All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

Western blot: Anti-Somatostatin receptor 2 antibody (ab134152, 1/1000). Lane 1: U937 lysates. Lane 2: INS-1 lysates. Lane 3: HEK-293 lysates (transfected)