Y-27632 dihydrochloride, Rho kinase inhibitor(AB120129)

2D chemical structure image of ab120129, Y-27632 dihydrochloride, Rho kinase inhibitor

A: Serum-starved human TM cells were incubated on chamber glass slides coated with recombinant human Gal8 in the presence of the ROCK inhibitor ab120129, at different concentrations. After 2 hr, cells were stained with rhodamine-labeled phalloidin, and cells with robust stress fibers were enumerated. Data are expressed as mean±SEM.

B: Cells were treated with an 120129 (Y27632) at 20 μM, stained with rhodamine-labeled phalloidin, and random fields were photographed. Note that cells treated with ab120129 are not spread and exhibit dendrite-like structures.

Credit: Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400

Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.

The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.