Western blot - HRP Anti-6X His tag® antibody (ab1187)

All lanes: Western blot - HRP Anti-6X His tag® antibody (ab1187) at 1/4000 dilution

Lane 1: 4ug Human His-tagged protein purified from E.coli

Lane 2: 2ug Human His-tagged protein purified from E.coli

Lane 3: 1ug Human His-tagged protein purified from E.coli

Lane 4: 0.5ug Human His-tagged protein purified from E.coli

Observed band size: 136 kDa