Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Rat liver mitochondria labeled with ab110413 (MS604). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.
Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4) labeling CI subunit NDUFB8 with ab110242 at 0.5 ug/ml.
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 μg -Lane 5) labeling CII-30kDa with ab14714 at 5 μg/mL. Secondary antibody is a goat anti-mouse antibody.
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Human skeletal muscle tissue lysate, (10 μg - Lane 1) and Ramos (human Burkitt's lymphoma cell line) whole cell lysate (10 μg - Lane 2) labeling CIII-Core protein 2 with ab14745 at 5 μg/mL. Secondary antibody is a goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), 1/3000 dilution.
Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (5 μg - Lane 4) labeling CIV subunit I with ab14705 at 0.5 μg/mL. Secondary antibody is a goat anti-mouse antibody.
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Isolated mitochondria from heart of human (10 μg - Lane 1), cow (4 μg - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 μg -Lane 5) labeling CV alpha subunit with ab14748 at 1 μg/mL
Western blot - Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Human liver tissue lysate, (10 μg - Lane 1) and HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (10 μg - Lane 2) labeling CV alpha subunit with ab14748 at 1 μg/mL. Secondary antibody is a goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), 1/3000 dilution.
