Total OXPHOS Rodent WB Antibody Cocktail(AB110413)

Rat liver mitochondria labeled with ab110413 (MS604). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.

Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4) labeling CI subunit NDUFB8 with ab110242 at 0.5 ug/ml.

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 μg -Lane 5) labeling CII-30kDa with ab14714 at 5 μg/mL. Secondary antibody is a goat anti-mouse antibody.

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

Human skeletal muscle tissue lysate, (10 μg - Lane 1) and Ramos (human Burkitt's lymphoma cell line) whole cell lysate (10 μg - Lane 2) labeling CIII-Core protein 2 with ab14745 at 5 μg/mL. Secondary antibody is a goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), 1/3000 dilution.

Isolated mitochondria from heart of human (5 μg - Lane 1), cow (1 ug - Lane 2), rat (10 μg - Lane 3), mouse (5 μg - Lane 4) labeling CIV subunit I with ab14705 at 0.5 μg/mL. Secondary antibody is a goat anti-mouse antibody.

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

Isolated mitochondria from heart of human (10 μg - Lane 1), cow (4 μg - Lane 2), rat (10 μg - Lane 3), mouse (10 μg - Lane 4), HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 μg -Lane 5) labeling CV alpha subunit with ab14748 at 1 μg/mL

Human liver tissue lysate, (10 μg - Lane 1) and HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (10 μg - Lane 2) labeling CV alpha subunit with ab14748 at 1 μg/mL. Secondary antibody is a goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP), 1/3000 dilution.