Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Liver samples from high fat diet (HFD) and standard carbohydrate diet (CHD) BALB/c and C57BL6/J mice were homogenised in an ALT assay buffer for the determination of ALT activity using ab105134. A separate batch of liver extracts was prepared and incubated in a buffer containing NP40 (5%) and supernatants containing the triglycerides were separated. Triglycerides concentration was determined on the supernatant fraction using ab65336. ALT activity and triglycerides concentration were determined by measuring OD at 570nm.
Waller-Evans H et al., PLoS One, 8(12). Fig 1d. doi: 10.1371/journal.pone.0082825 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.
Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575
Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Alanine transaminase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
Protein concentration for samples varied from 4 mg/mL to 13 mg/mL. Samples were diluted 9-27 fold and measured colorimetrically.
Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134)
Pyruvate measured colorimetrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.
