Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)(AB105134)

Liver samples from high fat diet (HFD) and standard carbohydrate diet (CHD) BALB/c and C57BL6/J mice were homogenised in an ALT assay buffer for the determination of ALT activity using ab105134. A separate batch of liver extracts was prepared and incubated in a buffer containing NP40 (5%) and supernatants containing the triglycerides were separated. Triglycerides concentration was determined on the supernatant fraction using ab65336. ALT activity and triglycerides concentration were determined by measuring OD at 570nm.

Waller-Evans H et al., PLoS One, 8(12). Fig 1d. doi: 10.1371/journal.pone.0082825 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.

Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575

Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

Alanine transaminase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.

Protein concentration for samples varied from 4 mg/mL to 13 mg/mL. Samples were diluted 9-27 fold and measured colorimetrically.

Pyruvate measured colorimetrically in cell lysate after 20 min and 40 min incubation time showing quantity (nmol) per 1 mln of tested cells.