PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®(AB102918)

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit Lightning-Link.

Robinson, Andrew P., et al used PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) as part of characterizing oligodendroglial populations. They used the kit to conjugate PE / R-Phycoerythrin to Mouse monoclonal anti-GALC antibody for use in flow cytometry.

SJL/J mice were immunized with PLP139-151 and scored daily for clinical disease. A cohort of SJL/J mice was sacrificed, and spinal cords were analyzed by flow cytometry (n=5). (A) Cells were distinguished from debris by forward and side scatter then singlet cells were gated. Live cells were gated by dead cell exclusion, and CNS resident cells were identified as CD45- or CD45low. (B) Oligodendroglial cells were defined by double positive staining: A2B5+PDGFRalpha+ early OPCs, A2B5+NG2+ intermediate OPCs, NG2+O4+ late OPCs, O4+MOG+ pre-myelinating oligodendrocytes, and GALC+MOG+ mature oligodendrocytes.

Image from Robinson, Andrew P., et al., PloS one, 9(9):e107649. doi: 10.1371/journal.pone.0107649. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling ADRB2 antibody for Flow Cytometry.

Bigler MB et al. used ab102918 to assess expression of the epinephrine receptor (ADRB2) on CD3- CD56+ lymphocytes by flow cytometry.

Image from Bigler MB et al., PloS One, 10(12):e0145635. Fig 3.; doi: 10.1371/journal.pone.0145635. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling F(ab')2 antibody for Bead array.

Ayoglu B et al. used ab102918 to detect complement activation driven C3 deposition on beads.

For measuring the background, classical, lectin & alternative and only alternative pathway activation, a serially diluted serum sample (1∶1–1∶160) was applied to empty, human IgG. The assay buffer for serum dilution contained either Ca2+-Mg2+, which promotes complement activation, or EDTA, which blocks complement activation. Plot displays for each serum dilution the respective median fluorescence intensity (MFI) value against varying concentrations of human IgG.

Image from Ayoglu B et al., PLoS One 9(5):e96403. Fig 2.; doi: 10.1371/journal.pone.0096403. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling anti-MUC1 SP antibodies FACS.

Kovjazin R et al. used ab102918 to evaluate the cell surface presence of the MUC1 SP domain by FACS.

The cell surface presence of the MUC1 SP domain was evaluated by gated FACS analysis on PC cells in fresh BM aspirates obtained from MM patients (A–E) and normal naive sample (F–H).

Image from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 3.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling SPmAb-6 antibody for Imagestream analysis.

Kovjazin R et al. used ab102918 for detection of MUC1 SP on the membrane of MUC1-positive tumor cells.

image from Kovjazin R et al., PLoS One, 9(1):e85400. Fig 2.; doi: 10.1371/journal.pone.0085400. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® labeling a panel of antibodies for Microsphere immunoassay based on Luminex.

Charlermroj R et al. used ab102918 as part of an experiment studying four different plant pathogens: a fruit blotch bacterium Acidovorax avenae subsp. citrulli (Aac), chilli vein-banding mottle virus (CVbMV, potyvirus), watermelon silver mottle virus (WSMoV, tospovirus serogroup IV) and melon yellow spot virus (MYSV, tospovirus).

(A-G) X-axis is antibody-coated microsphere and y-axis is median fluorescent intensity (MFI) from each RPE-labeled antibody. (H) Summary of selected antibody pair sets for the detection of the four plant pathogens.

Image from Charlermroj R et al., PloS One, 8(4):e62344. Fig 2.; doi: 10.1371/journal.pone.0062344. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/