Our advanced imaging system offers automated and high-throughput solutions for imaging and analyzing diverse cell types. The barcoded plates allow easy single-cell tracking for the formation of a colony over a period of time. The single-cell imager is also equipped with multichannel imaging for accurate single-cell detection and single-cell screening.

Live cell imaging is one of the microscopy techniques essential in biological and medical research for understanding the dynamics and behavior of individual cells in cell populations, such as their gene expression network, cell cycle, and cell signaling pathways. Available conventional methods (such as colorimetric MTT assays) for single-cell imaging are not enough to suit high-throughput applications, such as cell line development and monoclonality verification. They are time-consuming, laborious, and highly subjective processes.

Features

Product Highlights/Key Features of our Single Cell Imager

Our high-throughput single-cell imaging system comes with a host of features to help you with processing and analysis of single-cell:

• Our single-cell imager has white light and fluorescent imaging features that allow the generation of single-cell fluorescence images or images of different types based on the chosen imaging conditions. It captures live single-cell images in each well of 96- or 384-well plates with rapid acquisition times. Multichannel fluorescence imaging verifies the monoclonality of each cell from day zero and allows for comparative confluence assays, distinguishing between red and green signals.
• These devices track the measurements of each well automatically over time. The integrated advanced software automatically calculates confluence measurements and generates heatmaps, growth curves, and image montages for each well - thus streamlining multiple steps in cell line development workflow, starting from imaging and sample tracking to data analysis and report generation.
• Our imagers have the capability to screen and capture each well in two minutes. Additionally, they are compatible with a range of cell types, such as HEK, hybridomas, CHO, iPSCs, and other cell types.
• Our devices offer you high-resolution images of each well and allow you to track data and visualize images over time for efficient single-cell imaging data analysis.
• With just one hour of training, you can exploit our advanced high-throughput imaging software to automatically generate a growth curve, total growth, image montage, and mean rate for the cells under analysis. With time-lapse imaging, the software calculates confluence periodically.
• The Monoclonality Report feature simplifies the generation of regulatory documentation. The reports are automatically created according to your chosen parameters. This audit-ready document supports filing for an Investigational New Drug (IND) Application with the FDA (21 CFR Part 312).

Applications

Applications of Single-cell Imaging

Advances in single-cell imaging systems have allowed the easy visualization and identification of each cell in a population. From recognizing heterogeneity in a cell population to imaging subcellular structures, our automated imagers have revolutionized and streamlined research studies. They uncovered the power of single cells by combining high throughput image analysis and machine learning features.

• The fluorescent imager has extensive applications in investigating new cell lines or variants for optimizing clonal outgrowth strategies. Our automated imagers are compatible with any cell type, whether they be adherent or suspension cell types, such as IPSCs, HEK, CHO, hybridomas, and a few others.
• Our automated imagers have applications in the optimization of cell culture conditions and monoclonality verification. They help in the rapid assessment of cell line growth. Each well plate is analyzed and tracked automatically over a time period for real-time imaging and single-cell identification and analysis. Further, the device also displays cell confluence and cell number estimation, growth curve, and growth rate for each well.
• The imager offers image-driven proof of monoclonality from day zero. It focuses on wells with a single locus of growth and reviews image cell lineage for monoclonality confirmation using white light. This further validates the monoclonality of every single colony from the initial stage using fluorescence.
• Cell line development requires single-cell derived clones, which further needs the isolation of viable single-cells. This can be achieved using our automated single-cell imager that streamlines your cell biology workflows, enhances productivity, and offers more time for other lab tasks.
• Our imager streamlines your colony-forming assays by providing better insight into the growth rate, confluence, growth area, colony number, and growth curve for each well.
• Our comprehensive imagers are designed to help you perform label-free cell migration for studying the effect of a library of compounds and genes by offering fast imaging (<90 s per plate) and objective, automated cell migration analysis.

FAQs